vesicular glutamate transporter 2 135404 antibody  (Synaptic Systems)

Journal: The Journal of Physiology

Article Title: Vasopressin casts light on the suprachiasmatic nucleus

doi: 10.1113/JP274025

Figure Lengend Snippet: Primary antibodies used for retina and SCN immunohistochemistry

Article Snippet: Supplier Dilution Host eGFP AB3080 Millipore, UK 1:1000 Rabbit eGFP MAB3580 Millipore, UK 1:1000 Mouse Venus (GFP and YFP) ab13970 Abcam, UK 1:15000 Chicken tdTomato 362496 Clontech 1:500 Rabbit Gastrin releasing peptide ab43834 Abcam, UK 1:500 Rabbit Vasoactive intestinal peptide 20077 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Melanopsin AB19306 Abcam, UK 1:100 Rabbit Neuropeptide Y 22940 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Somatostatin 20067 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Vasopressin PS41 Professor H. Gainer (NIH, Bethesda, MD, USA) 1:1000 Mouse Vasopressin PC234L Merck Chemicals Ltd, UK 1:200 Rabbit Calbindin D‐28K 300 Swant, Switzerland 1:500 Mouse Calretinin 7699/3H Swant, Switzerland 1:500 Rabbit Parvalbumin 24428 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Glutamate decarboxylase 65/67 ADI‐MSA‐225 Enzo Life Sciences (UK) Ltd, Exeter, UK 1:1000 Mouse Vesicular glutamate transporter 2 135404 Synaptic Systems, Germany 1:1000 Guinea pig Glycine AB5020 Millipore, UK 1:100 Rabbit Tyrosine hydroxylase AB152 Millipore, UK 1:1000 Rabbit Dopamine β‐hyroxylase AB1585 Millipore, UK 1:2000 Rabbit Choline acetyltransferase AB144P Millipore, UK 1:1000 Goat Fos PC38 Millipore, UK 1:20000 Rabbit Fos 226003 Synaptic Systems, Germany 1:100000 Rabbit Open in a separate window Primary antibodies used for retina and SCN immunohistochemistry table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Secondary antibody and visualization Cat. no.

Techniques:

Journal: The Journal of Physiology

Article Title: Vasopressin casts light on the suprachiasmatic nucleus

doi: 10.1113/JP274025

Figure Lengend Snippet: A, flat mount of retina, focused on the ganglion cell layer (GCL), shows dispersed eGFP‐expressing cells (green cells); the blue staining is a nuclear marker DAPI, to show the location of all cells in the field of view; the inset shows the location of the image within the flat mount. B, eGFP‐cells occur in both the ganglion cell layer (GCL) and the inner nuclear layer (INL), as shown in a cross section of the flat mount. C, eGFP‐cells express vasopressin–neurophysin (VP‐NP); the successive images show fluorescence for eGFP, immunoreactive VP‐NP and overlaid images. D, PCR confirmation of expression of vasopressin mRNA (VP, 77 bp) and actin mRNA in the supraoptic nucleus (positive control) and the retina of wild‐type rats; the supraoptic nucleus contains magnocellular neurons that project to the posterior pituitary gland. Note there is no detectable oxytocin mRNA (OXT, 62 bp) in the retina; oxytocin is a closely related peptide that is also expressed in the supraoptic nucleus. E, eGFP‐cells (shown in a flat mount) co‐express the vesicle glutamate transporter vGLUT‐2 (white arrows) indicating that they use glutamate as a conventional neurotransmitter. F, some eGFP‐cells co‐express the photopigment melanopsin (white arrows; yellow arrow shows a cell immunopositive for melanopsin only).

Article Snippet: Supplier Dilution Host eGFP AB3080 Millipore, UK 1:1000 Rabbit eGFP MAB3580 Millipore, UK 1:1000 Mouse Venus (GFP and YFP) ab13970 Abcam, UK 1:15000 Chicken tdTomato 362496 Clontech 1:500 Rabbit Gastrin releasing peptide ab43834 Abcam, UK 1:500 Rabbit Vasoactive intestinal peptide 20077 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Melanopsin AB19306 Abcam, UK 1:100 Rabbit Neuropeptide Y 22940 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Somatostatin 20067 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Vasopressin PS41 Professor H. Gainer (NIH, Bethesda, MD, USA) 1:1000 Mouse Vasopressin PC234L Merck Chemicals Ltd, UK 1:200 Rabbit Calbindin D‐28K 300 Swant, Switzerland 1:500 Mouse Calretinin 7699/3H Swant, Switzerland 1:500 Rabbit Parvalbumin 24428 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Glutamate decarboxylase 65/67 ADI‐MSA‐225 Enzo Life Sciences (UK) Ltd, Exeter, UK 1:1000 Mouse Vesicular glutamate transporter 2 135404 Synaptic Systems, Germany 1:1000 Guinea pig Glycine AB5020 Millipore, UK 1:100 Rabbit Tyrosine hydroxylase AB152 Millipore, UK 1:1000 Rabbit Dopamine β‐hyroxylase AB1585 Millipore, UK 1:2000 Rabbit Choline acetyltransferase AB144P Millipore, UK 1:1000 Goat Fos PC38 Millipore, UK 1:20000 Rabbit Fos 226003 Synaptic Systems, Germany 1:100000 Rabbit Open in a separate window Primary antibodies used for retina and SCN immunohistochemistry table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Secondary antibody and visualization Cat. no.

Techniques: Expressing, Staining, Marker, Fluorescence, Positive Control

Journal: The Journal of Physiology

Article Title: Vasopressin casts light on the suprachiasmatic nucleus

doi: 10.1113/JP274025

Figure Lengend Snippet: A, scheme of the viral vector used to infect VP‐RGCs. B, intravitreal injection in wild‐type rats of an rAAV expressing Venus (green) under the control of the vasopressin promoter infects RGCs that express vasopressin–neurophysin (VP‐NP, red; yellow in overlay). C, Venus labelling in a retinal flat mount. D, dendrites of RGCs branching into the inner nuclear layer (INL) (D), or ganglion cell layer (GCL) (Di). E, Venus‐labelled axonal projections converge on the optic disc, where the optic nerve leaves the retina. F, enlarged in Fi, the VP‐RGCs project (green fibres; Venus) to the ventrolateral SCN; intrinsic vasopressin cells in red. G and H, retrograde tracer is found in some VP‐RGCs (Gi and Hi) after microinjection into the SCN (G; fluorogold), but not after injection into the superior colliculus (H, Fluoro‐Red beads). I and J, terminal boutons of Venus‐labelled fibres in the SCN express vGLUT‐2 and target vasoactive intestinal polypeptide (VIP; I) and gastrin‐releasing peptide (GRP; J) cells. The blue staining is a nuclear marker, DAPI. 3V, 3rd ventricle; ITR, inverted terminal repeat sequence; OC, optic chiasm.

Article Snippet: Supplier Dilution Host eGFP AB3080 Millipore, UK 1:1000 Rabbit eGFP MAB3580 Millipore, UK 1:1000 Mouse Venus (GFP and YFP) ab13970 Abcam, UK 1:15000 Chicken tdTomato 362496 Clontech 1:500 Rabbit Gastrin releasing peptide ab43834 Abcam, UK 1:500 Rabbit Vasoactive intestinal peptide 20077 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Melanopsin AB19306 Abcam, UK 1:100 Rabbit Neuropeptide Y 22940 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Somatostatin 20067 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Vasopressin PS41 Professor H. Gainer (NIH, Bethesda, MD, USA) 1:1000 Mouse Vasopressin PC234L Merck Chemicals Ltd, UK 1:200 Rabbit Calbindin D‐28K 300 Swant, Switzerland 1:500 Mouse Calretinin 7699/3H Swant, Switzerland 1:500 Rabbit Parvalbumin 24428 Immunostar, Newmarket Scientific, UK 1:500 Rabbit Glutamate decarboxylase 65/67 ADI‐MSA‐225 Enzo Life Sciences (UK) Ltd, Exeter, UK 1:1000 Mouse Vesicular glutamate transporter 2 135404 Synaptic Systems, Germany 1:1000 Guinea pig Glycine AB5020 Millipore, UK 1:100 Rabbit Tyrosine hydroxylase AB152 Millipore, UK 1:1000 Rabbit Dopamine β‐hyroxylase AB1585 Millipore, UK 1:2000 Rabbit Choline acetyltransferase AB144P Millipore, UK 1:1000 Goat Fos PC38 Millipore, UK 1:20000 Rabbit Fos 226003 Synaptic Systems, Germany 1:100000 Rabbit Open in a separate window Primary antibodies used for retina and SCN immunohistochemistry table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Secondary antibody and visualization Cat. no.

Techniques: Plasmid Preparation, Injection, Expressing, Control, Microinjection, Staining, Marker, Sequencing